How to Inject Peptides: Subcutaneous Technique

Insulin syringes (29–31 gauge, 5/16 inch), bacteriostatic water for reconstitution, alcohol prep pads, a sharps container, and the lyophilized vial.

Use bacteriostatic water — not sterile water — for any vial you will access more than once. The benzyl alcohol preservative is what makes repeat entry safe.

Swab both stoppers, draw your diluent, and let it run down the vial wall rather than jetting directly onto the powder.

Swirl gently until clear. Never shake — mechanical shear can denature the peptide. Store reconstituted vials refrigerated at 2–8°C.

Choose abdomen (2 inches from the navel), outer thigh, or the back of the arm. Rotate sites each dose to prevent lipohypertrophy.

Pinch the skin, insert at 45–90 degrees, inject slowly, count three seconds, withdraw, and apply light pressure without rubbing.

Dispose of the needle immediately in a sharps container. Never recap or reuse.

Spreading redness, warmth, or fever suggests infection. Hives, throat tightness, or wheezing suggest an allergic reaction and require emergency care.